Binary vector pcambia-1300

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WebApr 7, 2024 · How to order pCAMBIA 1300 vector? Question. 3 answers. Apr 3, 2024; ... The steps include cloning your gene into a binary vector, transferred the vector into Agrobacterium, ... WebProduct name pCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for …

Schematic diagram of the pCAMBIA 1304 plasmid. …

WebSep 8, 2014 · The fragment was then ligated with a GFP-containing pCAMBIA 1300 binary vector to develop the vector named as pDsTGFP (Figure 1B). The vector has two AhdI sites at the 13 th and 1329 th position, respectively (Figure 1B). Thus, the 3′ T-overhang ends can be generated by digesting the pDsTGFP vector with the enzyme AhdI (Figure … WebBinary vector pCAMBIA 1301 showing hptII gene near left border and GUS gene near right border of T-DNA from publication: An Efficient Protocol for the Agrobacterium-mediated Genetic Transformation ... group calgary orthodontics

Typical structure of a binary vector. Key components and their …

Webthe binary vector pCambia 1300-Rb:: uidA to replace the uidA gene and generate pCambia 1300-Rb::MCM2 fragment2. The M2-sense1 primer allowed us to add an XbaI restriction site at the 5 ′ end of the PCR product, and M2-antisense1 was designed downstream of the internal XbaI (1844 bp down-stream of the start codon) site. The … Web作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。. 通过Google搜索"Biovector+您所需 … WebpCAMBIA1300 Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance genes. Sequence Author: Cambia Open in SnapGene Try … film counter

pCambia1300 Plant Expression Vector (ab275754) Abcam

The Arabidopsis MCM2 gene is essential to embryo …

WebBinary vector pCAMBIA-1300, complete sequence Binary vector pCAMBIA-1300, complete sequence. Your browsing activity is empty. Activity recording is turned off. Turn … WebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … film country indiaWebDec 18, 2008 · Vector Construction A transformation vector was constructed and denoted pCHO. It was derived from the Agrobacterium tumefaciens binary vector pCAMBIA 1300 ( http://www.cambia.org.au) by replacement of the original hph cassette driven by the 35S promoter from CaMV with newly constructed cassettes. group calendar sharepoint webpart

"WebpCAMBIA has wide range of vector like 1300,2300,2301. it is binary vector kanamycin based with small replicon and has plant selection … " - Binary vector pcambia-1300

Binary vector pcambia-1300

A Novel Binary T-Vector with the GFP Reporter Gene for Promoter …

WebNov 25, 2024 · In addition, we tested the pCAMBIA 1300-PtHCA 2-1 promoter-GUS binary expression vector, which contains the GUS gene ORF under the control of Populus … WebSep 21, 2024 · A genetically engineered chimeric virus crTMV-CP-PLRV composed of the crucifer-infecting tobacco mosaic virus (crTMV) RNA and the potato leafroll virus (PLRV) coat protein (CP) was obtained by agroinfiltration of Nicotiana benthamiana with the binary vector pCambia-crTMV-CPPLRV. The significant levels of the chimeric virus enabled …

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WebAug 14, 2015 · Grains of an insect resistant transgenic Bt rice (Oryza sativa L. Japonica cv Ariete), transformed by Agrobacterium tumefaciens with the binary vector pCAMBIA 1300 containing the synthetic Cry1B gene from Bacillus thuringiensis, and its corresponding wild-type (WT) were used in this study . The CRM of the GM maize MON863 9.85 % (ERM … WebEnter the email address you signed up with and we'll email you a reset link.

WebA twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase (hpt) marker gene. WebClassification and research data for Binary vector pCAMBIA-1300, unclassified. Classification and research data for Binary vector pCAMBIA-1300, unclassified. Skip to …

WebpCAMBIA1302. Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GFP genes. Sequence Author: Cambia. Open in … WebPcambia 1300 (Search Vector Database) Vector type Plant Expression Selectable markers Hygromycin Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name FLAG-Pup (E) Promoter 35S Tag / Fusion Protein Triple Flag Tag …

WebThe cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants.

WebJun 1, 2007 · The cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants. group calendar sharepoint onlineWebBinary vector pCAMBIA-1300 NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 1 (Standard) Plastid genetic code: Translation table 11 (Bacterial, … film country lifeWebA. tumefaciens strains EHA 101 and EHA 105 contained the disarmed plasmid pCAMBIA 1304 which contains genes for hygromycin (hyg), β-gluconidase (gusA) and gfp (Fig. 2). The DHα E.coli strain ... group calendar vs team calendarWebNov 25, 2024 · Agrobacterium tumefaciens strain GV3101 was transformed with the pBI121, pCAMBIA 1300, and recombinant pCAMBIA-PTHCA 2-1 Promoter-GUS binary vectors ( Supplementary Figure S1 ). The pBI121 … group calendars in teamsWebJun 9, 2024 · In the first step, an EcoRI/KpnI fragment of ~ 2.4 kb was excised from the plasmid pKSMYMIV-A and cloned into the EcoRI/KpnI sites of binary vector pCAMBIA-1300 (Cambia) to produce the recombinant plasmid pCAM-MYMIV-AM. film countrymanWebThe pBIN19 vector was developed in the 1980s and is one of the first and most widely used binary vectors. The pGreen vector, which was developed in 2000, is a newer version of … group calendar with approval for vacationWebpCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system. The pCambia1300 vector offers: High copy number in E.coli for high DNA yields group calgary